| 教授 性別: 女
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研究興趣
藥物轉(zhuǎn)運蛋白的結(jié)構(gòu)-功能關(guān)系
藥物轉(zhuǎn)運蛋白在獸藥吸收、分布和排除(ADME)中的作用及其調(diào)控
膜蛋白在對外界環(huán)境改變的反應中的信號傳導作用
學歷
1998.9-2003.12 羅格斯大學植物學與植物病理學系 (Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Jersey, USA), 博士
1992.9-1996.7 中山大學化學系,學士
工作經(jīng)歷
2009.7月至今 華南農(nóng)業(yè)大學生命科學學院 教授
2007.7-2009.6 哥倫比亞大學醫(yī)學院輻射研究中心 (Center for Radiological Research, Columbia University Medical Center, New York, NY, USA) 博士后研究科學家
2006.12-2007.6 勞倫斯伯克萊國家實驗室生物學分部 (Life science division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA) 訪問博士后學者
2003.12-2006.12 羅格斯大學藥學院藥學系 (Department of Pharmaceutics, College of Pharmacy, Rutgers, The State University of New Jersey, New Jersey, USA) 博士后/助理研究員/副研究員
講授課程
本科生授課：
動物生物化學 （雙語授課）
生物化學A（雙語授課）
生物化學C
生物化學D（雙語授課）
生物化學與分子生物學（雙語授課）
生化與分子生物學專題
生物技術(shù)進展
生物專業(yè)英語
研究生授課：
生物化學與分子生物學專業(yè)英語
生物化學與分子生物學文獻綜述
生化與分子生物學進展
高級生物化學
1.人體轉(zhuǎn)運蛋白的結(jié)構(gòu)-功能關(guān)系研究
藥物轉(zhuǎn)運蛋白和藥物代謝酶，藥物目標的共同作用決定了人體對許多藥物的反應。因此，藥物轉(zhuǎn)運是生物體對藥物吸收的關(guān)鍵環(huán)節(jié)。轉(zhuǎn)運蛋白功能的缺失，會造成人體藥物排除或解毒障礙，從而造成藥物誘導的肝或腎衰竭。近年來，由于認識到轉(zhuǎn)運蛋白在藥物轉(zhuǎn)運中的重要作用，許多大型跨國藥廠已把對這類蛋白在藥物動力學和藥效學上的研究作為藥物研發(fā)中的重要一環(huán)。然而，迄今為止，對它們的結(jié)構(gòu)-功能關(guān)系的研究只有不多的報道。本實驗室利用各種生化技術(shù)，開展對藥物轉(zhuǎn)運蛋白結(jié)構(gòu)-功能關(guān)系的系統(tǒng)研究，鑒定其序列中起關(guān)鍵作用的氨基酸，探索它們翻譯后調(diào)節(jié)控制機理。
2.藥物轉(zhuǎn)運蛋白在獸藥吸收和排除中的作用
隨著畜牧業(yè)的現(xiàn)代化、集約化和規(guī)模化生產(chǎn)，獸藥及獸藥添加劑在降低發(fā)病率與死亡率、提高飼料利用率、促進生長和改善肉品品質(zhì)方面起著十分顯著的作用，已成為現(xiàn)代畜牧業(yè)發(fā)展不可缺少的物質(zhì)基礎。但是，由于飼養(yǎng)技術(shù)的落后和經(jīng)濟利益的驅(qū)使，養(yǎng)殖過程中濫用獸藥和超標使用獸藥的現(xiàn)象普遍存在。各種藥物及激素殘留對于人體健康和生態(tài)環(huán)境所帶來的不良影響日益引起關(guān)注。對藥物的吸收效率，是由吸收轉(zhuǎn)運蛋白和外排轉(zhuǎn)運蛋白協(xié)同作用而共同決定的。通過系統(tǒng)研究轉(zhuǎn)運蛋白在食品動物藥物吸收和排泄中的作用，將能填補吸收轉(zhuǎn)運蛋白在獸藥生物利用度中所起的作用方面的空白，為有效地提高獸藥的吸收分布提供重要的理論依據(jù)。
3. 膜蛋白在信號傳導中的作用
膜蛋白是否能正確地表達在特定的膜結(jié)構(gòu)中是其發(fā)揮正常功能的關(guān)鍵因素，膜蛋白的翻譯后修飾，在細胞器如內(nèi)質(zhì)網(wǎng)、高爾基體中的處理過程受多種因素的影響。細胞通過調(diào)控膜蛋白在細胞膜表面的表達量和表達位點，對各種外界環(huán)境變化（如營養(yǎng)元素量的改變、外界刺激等）作出反應。實驗室的另一研究方向是以生物化學和細胞生物學方法，研究膜蛋白在信號傳導過程的重要作用，探索其所介導的細胞對外界刺激如輻射、環(huán)境毒物的反應。
發(fā)表論文
專著章節(jié)：
Hong, M., You, G. (2007) Regulation of drug transporter activity (chapter 17, pp517-533) in Drug Transporters: Molecular Characterization and Role in Drug Disposition. Eds Guofeng You, Marilyn Morris and Binghe Wang. John Wiley & Son Inc
論文：
Wu Z, Yuan J, Li K, Wang X, Zhang Z, Hong M* (2024)The induction of drug uptake transporter OATP1A2 by radiation is mediated by non-receptor tyrosine kinase YES-1. Drug Metab Dispos. 52(11):1244-1252.
Wang C, Yang J, Xu Z, Lv L, Chen S, Hong M, Liu JH (2024) Promoter regulatory mode evolution enhances the high multidrug resistance of tmexCD1-toprJ1. mBio. 15(5):e0021824
Ni C, Hong M* (2024) Oligomerization of drug transporters: Forms, functions, and mechanisms. Acta Pharm Sin B. 14(5):1924-1938.
Zhou W, Chen Y, Zheng Y, Bai Y, Yin J, Wu XX, Hong M, Liang L, Zhan GJ, Gao Y, Sun N, Li J, Zhang Y, Wu L, Jin X, Niu J* (2024) Characterizing immune variation and diagnostic indicators of preeclampsia by single-cell RNA sequencing and machine learning. Commun Biol, 7(1):32.
Wang X, Hong M* (2023) Protein kinases and cross-talk between post- translational modifications in the regulation of drug transporters. Mol Pharmacol 103(1):9-20.
Zhang Z, Li K, Hong M* (2022) Radiation-induced bystander effect and cytoplasmic irradiation studies with microbeams. Biology-Basel, 11(7):945
Chen J, Xue Y, Shuai X, Ni C, Fang Z, Ye L, Hong M* (2022) Effect of major components of Tripterygium wilfordii Hook.f on the uptake function of organic anion transporting polypeptide 1B1. Toxicology and Applied Pharmacology. 435:115848
Ni C, Wang X, Chen J, Xu S, Ye W, Hong M* (2021) Leucine heptad motifs within transmembrane domains affect function and oligomerization of human organic anion transporting polypeptide 1B1. Biochim Biophys Acta Biomembr 2021 Apr 1;1863(4):183554. doi: 10.1016/j.bbamem.2021.183554. Epub 2021 Jan 8.
Fang Z, Wang Y, Wang Z, Xu M, Ren S, Yang D, Hong M*, Xie W* (2020) ERINA is an estrogen-responsive lncRNA that drives breast cancer through the E2F1/RB1 pathway.Cancer Res. 80(20):4399-4413.
Wang Y, Fang Z, Hong M, Yang D, Xie W* (2020) Long-noncoding RNAs (lncRNAs) in drug metabolism and disposition, implications in cancer chemo-resistance. Acta Pharmaceutica Sinica B 10(1):105-112.
Xu S, Chen G, Liu Z, Xu D, Wu Z, Li Z, Hong M* (2019) Site-directed mutagenesis reveals crucial residues in Escherichia coli Resistance-Nodulation-Division efflux pump OqxB.Microb Drug Resist.26(6):550-560. [Epub ahead of print]
Lin X, Xiang Z, Wang B, Chen H, Zhou T, Hong M* (2019) Interaction of swine organic anion transporting polypeptide 1a2 with tetracycline, macrolide and β-lactam antibiotics. Toxicol Appl Pharmacol. 379:114649.
Wang X, Chen J, Xu S, Ni C, Fang Z, Hong M* (2019) Amino-terminal region of human organic anion transporting polypeptide 1B1 dictates transporter stability and substrate interaction. Toxicol Appl Pharmacol. 378:114642.
Wang X, Liang Y, Fang Z, Huang J, Hong M* (2019) The intracellular NPxY motif is critical in maintaining the function and expression of human organic anion transporting polypeptide 1B1. Biochim Biophys Acta Biomembr.1861(6):1189-1196.
Fang Z, Huang J, Chen J, Xu S, Xiang Z, Hong M* (2018)Transmembrane domain 1 of human organic anion transporting polypeptide 2B1 is essential for transporter function and stability.Mol Pharmacol. 94(2):842-849.
Xiang Z, Li W, Wang L, Yi J, Chen K, Hong M* (2018) Identification of a NFκB inhibition site on the proximal promoter region of human organic anion transporting polypeptide 1A2 coding gene SLCO1A2.Drug Metab Dispos. 46(5):643-651.
Zhou C, Rong Y, Konishi T, Xiang Z, Zihui F, Hong M*（2017）Effect of carbon-ion radiation on drug transporters organic anion transporting polypeptides in breast cancer cells. Radiat Res. 187(6):689-700.
Ni C, Yu X, Fang Z, Huang J, Hong M*(2017) Oligomerization study of human organic anion transporting polypeptide 1B1.Mol Pharmceutic.14(2):359- 367.
Hong M (2017) Biochemical studies on the structure-function relationship of major drug transporters in the ATP-binding cassette family and solute carrier family. Adv Drug Deliv Rev. 116(2017): 3-20.
Fang Z, Xu A, Wu L, Hei TK, Hong M* (2016) The role of protein kinase C alpha translocation in radiation-inducedbystander effect. Sci Rep. 6:25817.
Hong W, Wu Z, Fang Z, Huang J, Huang H, Hong M* (2015) Amino acid residues in the putative transmembrane domain 11 of human organic anion transporting polypeptide 1B1 dictate transporter substrate binding, stability, and trafficking. Mol Pharmaceut.12(12):4270-6.
Hong M*, Hong W, Ni C, Huang J, Zhou C.(2015) Protein kinase C affects the internalization and recycling of organic anion transporting polypeptide 1B1. Biochim Biophys Acta. 1848(10 Pt A):2022-2030.
Hu W, Xu S, Yao B, Hong M,Wu X, Pei H, Chang L, Ding N, Gao X, Ye C, Wang J, Hei TK, Zhou G.（2014）MiR-663 inhibits radiation-induced bystander effects by targeting TGFB1 in a feedback mode. RNA Biol. 11(9):1189-98
Xiao Y, Deng J, Liu X, Huang J, Sun Y, Dai R, Hong M * (2014) Different binding sites of bovine organic anion-transporting polypeptide1a2 are involved in the transport of different fluoroquinolones. Drug Metab Dispos. 42(8):1261-7.
Hong M. (2014) Heavy ion-related bystander effects. Rend. Fis. Acc. Lincei 25(1 supp): 65-68.
Hong M. (2014) Critical domains within the sequence of human organic anion transporting polypeptides. Curr Drug Metab.15(3):265-70.
Huang J, Li N, Hong W, Zhan K, Yu X, Huang H, Hong M.* (2013) Conserved tryptophan residues within putative transmembrane domain 6 affect transport function of organicaniontransportingpolypeptide 1B1.Mol Pharmacol. 84(4):521-7.
Zhan K, Yao J, Huang J, Zhou G, Li W, Chen L, Hong M* (2013) Effect of X-ray irradiation on expression of organic anion transporting polypeptides. Int J Radiat Biol. 89(10):832-40 .
Liu, X, Huang, J, Sun, Y, Zhan, K, Zhang, Z, Hong, M* (2013) Identification of multiple binding sites for substrate transport in bovine organic anion transporting polypeptide 1a2. Drug Metab Dispos, 41(3):602-607.
Yu, Y, Liu, X, Zhang, Z, Xiao, Y, Hong, M* (2013) Cloning and functional characterization of the swine (Sus scrofa) organic anion transporting polypeptide 1a2. Xenobiotica. 43(8):738-44.
Yao J，Hong, W, Huang, J, Zhan K, Huang H, Hong, M* (2012) N-glycosylation dictates proper processing of organic anion transporting polypeptide 1B1. PLoS One.7(12): e52563.
Li N, Hong W, Huang H, Lu H, Lin G, Hong M* (2012) Identification of amino acids essential for estrone-3-sulfate transport within transmembrane domain 2 of organic anion transporting polypeptide 1B1.PLoS One. 7(5):e36647.
